Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis
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چکیده
منابع مشابه
Research Note Recombinant Expression of Toxocara Canis Excretory-secretory Antigen Tes-120 in Escherichia Coli
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recomb...
متن کاملSerodiagnosis of Human Toxocariasis Using Adult Antigens of Toxocara canis
Introduction Toxocara canis has a worldwide distribution and is regarded as the main cause of human toxocariasis (1, 2). Infection in humans, particularly children, is frequently caused by accidental ingestion of embryonated Toxocara eggs present in soil, water, food, dirty hands, and vegetables or by ingestion of larvae in under-cooked giblets (3). The disease can be asymptomatic or can lead t...
متن کاملSerodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae
Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses T. canis excretory-secretory antigen to detect specific IgG antibodies by ELISA. We investigated the serodiagnostic efficacy of ELISA using crude antigen of T. canis larvae (TCLA). Serum specimens of 64 clin...
متن کاملIdentification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis.
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we f...
متن کاملDevelopment of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis.
The specificity of the recombinant Toxocara canis antigen developed for the immunodiagnosis of human toxocariasis was compared with that of the excretory-secretory antigen from T. canis second-stage larvae (TES) by enzyme-linked immunosorbent assay. A total of 153 human serum samples from patients infected with 20 different helminths, including 11 cases of toxocariasis, were examined. No false-...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2015
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.15-0190